{"product_id":"proteintech-66155-1-ig","title":"Proteintech, 66155-1-Ig, NUMBL Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe NUMBL (66155-1-Ig) by Proteintech is a Monoclonal antibody targeting NUMBL in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse samples\n66155-1-Ig targets NUMBL in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: L02 cells\nPositive IHC detected in: mouse brain tissue, human gliomas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: PC-12 cells, L02 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nNumbl (also known as numblike or Nbl), as a conserved homolog of Drosophila Numb, is specfically expressed in the brain. Numbl is a cytoplasm protein and has redundant functions in embryonic neurogenesis. It plays an important role in ependymal wall integrity and subventricular zone neuroblast survival. However, its expression and function in the central nervous system lesion are still unclear.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nHost \/ Isotype: Mouse \/ IgG2a\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag16771 Product name: Recombinant human NUMBLIKE protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-280 aa of BC001794 Sequence: MNKLRQSLRRRKPAYVPEASRPHQWQADEDAVRKGTCSFPVRYLGHVEVEESRGMHVCEDAVKKLKAMGRKSVKSVLWVSADGLRVVDDKTKDLLVDQTIEKVSFCAPDRNLDKAFSYICRDGTTRRWICHCFLALKDSGERLSHAVGCAFAACLERKQRREKECGVTAAFDASRTSFAREGSFRLSGGGRPAEREAPDKKKAEAAAAPTVAPGPAQPGHVSPTPATTSPGEKGEAGTPVAAGTTAAAIPRRHAPLEQLVRQGSFRGFPALSQKNSPFKR Predict reactive species\nFull Name: numb homolog (Drosophila)-like\nCalculated Molecular Weight: 65 kDa\nObserved Molecular Weight: 70 kDa\nGenBank Accession Number: BC001794\nGene Symbol: NUMBL\nGene ID (NCBI): 9253\nRRID: AB_2881551\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q9Y6R0\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888307130537,"sku":"66155-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-66155-1-ig","provider":"Iright","version":"1.0","type":"link"}