{"product_id":"proteintech-66219-1-ig","title":"Proteintech, 66219-1-Ig, N-cadherin Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe N-cadherin (66219-1-Ig) by Proteintech is a Monoclonal antibody targeting N-cadherin in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse, rat, pig, rabbit samples\n66219-1-Ig targets N-cadherin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: rabbit brain tissue, pig brain tissue,  human heart tissue,  MKN-45 cells,  HEK-293 cells,  PC-3 cells,  mouse brain tissue,  rat brain tissue,  C2C12 cells,  C6 cells,  Saos-2 cells,  SGC-7901 cells,  ROS1728 cells,  NIH\/3T3 cells\nPositive IHC detected in: mouse heart tissue, mouse brain tissue,  human malignant melanoma tissue,  human ovary tumor tissue,  rat heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: mouse heart tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:7500-1:30000\nImmunofluorescence (IF)-P: IF-P : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat, pig, rabbit\nCited Reactivity: human, mouse, rat, pig, canine, monkey\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag4996 Product name: Recombinant human N-cadherin protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 33-350 aa of BC036470 Sequence: KTGFPEDVYSAVLSKDVHEGQPLLNVKFSNCNGKRKVQYESSEPADFKVDEDGMVYAVRSFPLSSEHAKFLIYAQDKETQEKWQVAVKLSLKPTLTEESVKESAEVEEIVFPRQFSKHSGHLQRQKRDWVISPINLPENSRGPFPQELVRIRSDRDKNLSLRYSVTGPGADQPPTGIFIINPISGQLSVTKPLDREQIARFHLRAHAVDINGNQVENPIDIVINVIDMNDNRPEFLHQVWNGTVPEGSKPGTYVMTVTAIDADDPNALNGMLRYRIVSQAPSTPSPNMFTINNETGDIITVAAGLDREKVQQYTLIIQ Predict reactive species\nFull Name: cadherin 2, type 1, N-cadherin (neuronal)\nCalculated Molecular Weight: 906 aa, 100 kDa\nObserved Molecular Weight: 130 kDa\nGenBank Accession Number: BC036470\nGene Symbol: N-cadherin\nGene ID (NCBI): 1000\nRRID: AB_2881610\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: P19022\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887969390761,"sku":"66219-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-66219-1-ig","provider":"Iright","version":"1.0","type":"link"}