{"product_id":"proteintech-66283-1-ig","title":"Proteintech, 66283-1-Ig, IGF1R\/CD221 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe IGF1R\/CD221 (66283-1-Ig) by Proteintech is a Monoclonal antibody targeting IGF1R\/CD221 in WB, IHC, ELISA applications with reactivity to human, rat samples\n66283-1-Ig targets IGF1R\/CD221 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: MCF-7 cells, HepG2 cells,  HeLa cells,  HUVEC cells,  A549 cells,  HEK-293 cells,  ROS1728 cells,  C6 cells\nPositive IHC detected in: human breast cancer tissue,  human endometrial cancer tissue,  human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:8000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nIGF1R, also named as CD221, is a receptor tyrosine kinase which mediates actions of INS-like growth factor 1 (IGF1). IGF1R binds IGF1 with high affinity and IGF2 and INS with a lower affinity, the activated IGF1R is involved in cell growth and survival control. It is crucial for tumor transformation and survival of malignant cells. IGF1R is translated as ~220 kDa pro-receptors (PMID: 33969359). IGF1R is a heterotetrameric receptor, composed of two alpha subunits (~130 kDa) and two beta subunits (~100-120 kDa) (PMID: 6279656). Both the alpha and beta subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: IGF I receptor\nCalculated Molecular Weight: 155 kDa\nObserved Molecular Weight: 130-140 kDa\nGenBank Accession Number: NM_000875\nGene Symbol: IGF1R\nGene ID (NCBI): 3480\nRRID: AB_2881666\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: P08069\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888003666089,"sku":"66283-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-66283-1-ig","provider":"Iright","version":"1.0","type":"link"}