{"product_id":"proteintech-66347-1-ig","title":"Proteintech, 66347-1-Ig, SIX2 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe SIX2 (66347-1-Ig) by Proteintech is a Monoclonal antibody targeting SIX2 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat, pig, canine samples\n66347-1-Ig targets SIX2 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, canine samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: pig skeletal muscle tissue, HEK-293 cells,  MDCK cells,  rat skeletal muscle tissue,  NCCIT cells,  L02 cells,  mouse skeletal muscle tissue\nPositive IHC detected in: mouse embryo tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene \n\nfamily have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in \n\nadult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing \nmesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599].\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat, pig, canine\nCited Reactivity: human, mouse\nHost \/ Isotype: Mouse \/ IgG2b\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag2124 Product name: Recombinant human SIX2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-291 aa of BC024033 Sequence: MSMLPTFGFTQEQVACVCEVLQQGGNIERLGRFLWSLPACEHLHKNESVLKAKAVVAFHRGNFRELYKILESHQFSPHNHAKLQQLWLKAHYIEAEKLRGRPLGAVGKYRVRRKFPLPRSIWDGEETSYCFKEKSRSVLREWYAHNPYPSPREKRELTEATGLTTTQVSNWFKNRRQRDRAAEAKERENNENSNSNSHNPLNGSGKSVLGSSEDEKTPSGTPDHSSSSPALLLSPPPPGLPSLHSLGHPPGPSAVPVPVPGGGGADPLQHHHGLQDSILNPMSANLVDLGS Predict reactive species\nFull Name: SIX homeobox 2\nCalculated Molecular Weight: 291 aa, 32 kDa\nObserved Molecular Weight: 37 kDa\nGenBank Accession Number: BC024033\nGene Symbol: SIX2\nGene ID (NCBI): 10736\nRRID: AB_2881727\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q9NPC8\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888008089769,"sku":"66347-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-66347-1-ig","provider":"Iright","version":"1.0","type":"link"}