{"product_id":"proteintech-66410-1-ig","title":"Proteintech, 66410-1-Ig, SIRT2 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe SIRT2 (66410-1-Ig) by Proteintech is a Monoclonal antibody targeting SIRT2 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human,  mouse,  rat,  pig samples\n66410-1-Ig targets SIRT2 in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human,  mouse,  rat,  pig samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: LNCaP cells, fetal human brain tissue,  pig heart tissue,  pig brain tissue,  rat heart tissue,  rat brain tissue,  mouse brain tissue,  MCF-7 cells,  HeLa cells,  HEK-293 cells,  Jurkat cells,  K-562 cells\nPositive IHC detected in: human kidney tissue,  human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe silent information regulator(SIR2) family of genes is highly conserved from prokaryotes to eukaryotes and is involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA damage repair, and aging. SIR2 contains a 323 amino acid catalytic core domain with a NAD-binding domain and a large groove which is the likely site of catalysis. SIR2 is widely expressed and highly expressed in the heart, brain, and skeletal muscle, while it is weakly expressed in the placenta and lungs. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of the microtubule network. SIRT2 exists in various isoforms and the molecular weight of isoforms are 35 kDa, 40 kDa, and 42 kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat, pig\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag7756 Product name: Recombinant human SIRT2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-352 aa of BC003547 Sequence: MDFLRNLFSQTLSLGSQKERLLDELTLEGVARYMQSERCRRVICLVGAGISTSAGIPDFRSPSTGLYDNLEKYHLPYPEAIFEISYFKKHPEPFFALAKELYPGQFKPTICHYFMRLLKDKGLLLRCYTQNIDTLERIAGLEQEDLVEAHGTFYTSHCVSASCRHEYPLSWMKEKIFSEVTPKCEDCQSLVKPDIVFFGESLPARFFSCMQSDFLKVDLLLVMGTSLQVQPFASLISKAPLSTPRLLINKEKAGQSDPFLGMIMGLGGGMDFDSKKAYRDVAWLGECDQGCLALAELLGWKKELEDLVRREHASIDAQSGAGVPNPSTSASPKKSPPPAKDEARTTEREKPQ Predict reactive species\nFull Name: sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)\nCalculated Molecular Weight: 43 kDa\nObserved Molecular Weight: 37-45 kDa\nGenBank Accession Number: BC003547\nGene Symbol: SIRT2\nGene ID (NCBI): 22933\nRRID: AB_2881782\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: Q8IXJ6\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887990821033,"sku":"66410-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-66410-1-ig","provider":"Iright","version":"1.0","type":"link"}