{"product_id":"proteintech-66515-1-ig","title":"Proteintech, 66515-1-Ig, E2F1 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe E2F1 (66515-1-Ig) by Proteintech is a Monoclonal antibody targeting E2F1 in WB, ELISA applications with reactivity to human, rat samples\n66515-1-Ig targets E2F1 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: LNCaP cells, HepG2 cells,  HeLa cells,  HEK-293 cells,  Jurkat cells,  HSC-T6 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nTranscription factor E2F1 (E2F1), also known as RBBP3, is a transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1\/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. The calculated molecular weight of E2F1 is 47 kDa, but the sumoylated E2F1 is bout 55-60 kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Mouse \/ IgG2b\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag17363 Product name: Recombinant human E2F1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 88-438 aa of BC050369 Sequence: VKRRLDLETDHQYLAESSGPARGRGRHPGKGVKSPGEKSRYETSLNLTTKRFLELLSHSADGVVDLNWAAEVLKVQKRRIYDITNVLEGIQLIAKKSKNHIQWLGSHTTVGVGGRLEGLTQDLRQLQESEQQLDHLMNICTTQLRLLSEDTDSQRLAYVTCQDLRSIADPAEQMVMVIKAPPETQLQAVDSSENFQISLKSKQGPIDVFLCPEETVGGISPGKTPSQEVTSEEENRATDSATIVSPPPSSPPSSLTTDPSQSLLSLEQEPLLSRMGSLRAPVDEDRLSPLVAADSLLEHVREDFSGLLPEEFISLSPPHEALDYHFGLEEGEGIRDLFDCDFGDLTPLDF Predict reactive species\nFull Name: E2F transcription factor 1\nCalculated Molecular Weight: 437 aa, 47 kDa\nObserved Molecular Weight: 55-60 kDa\nGenBank Accession Number: BC050369\nGene Symbol: E2F1\nGene ID (NCBI): 1869\nRRID: AB_2881878\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q01094\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887976829097,"sku":"66515-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-66515-1-ig","provider":"Iright","version":"1.0","type":"link"}