{"product_id":"proteintech-66516-1-ig","title":"Proteintech, 66516-1-Ig, SMAD3 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe SMAD3 (66516-1-Ig) by Proteintech is a Monoclonal antibody targeting SMAD3 in WB, IHC, ELISA applications with reactivity to human, mouse samples\n66516-1-Ig targets SMAD3 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HT-1080 cells, NIH\/3T3 cells,  A431 cells,  HEK-293 cells,  Jurkat cells,  K-562 cells,  4T1 cells\nPositive IHC detected in: human stomach cancer tissue, mouse lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:12000\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. SMAD3 localizes in the nucleus and cytoplasm. SMAD3 plays an essential role in development and maintenance of self-tolerance and is a critical mediator of the TGFB signaling pathway. SMAD3 is involved in TGFB dependent regulation of steroidogenesis and in T-cell response to TGFB.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse, pig, rabbit\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: SMAD family member 3\nCalculated Molecular Weight: 48 kDa\nObserved Molecular Weight: 55-60 kDa\nGenBank Accession Number: NM_005902\nGene Symbol: SMAD3\nGene ID (NCBI): 4088\nRRID: AB_2881879\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: P84022\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887971946665,"sku":"66516-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-66516-1-ig","provider":"Iright","version":"1.0","type":"link"}