{"product_id":"proteintech-66570-1-ig","title":"Proteintech, 66570-1-Ig, TLR5 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe TLR5 (66570-1-Ig) by Proteintech is a Monoclonal antibody targeting TLR5 in WB, IF\/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat, pig samples\n66570-1-Ig targets TLR5 in WB, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A549 cells, THP-1 cells,  rat spleen tissue,  HeLa cells,  RAW 264.7 cells\nPositive IF\/ICC detected in: THP-1 cells\nPositive FC (Intra) detected in: THP-1 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:400-1:1600\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nTLR5 belongs to the Toll-like receptor family which are important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR5 is known to recognize bacterial flagellin from invading mobile bacteria. TLR5 acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Mutations in TLR5 gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat, pig\nCited Reactivity: mouse\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag13840 Product name: Recombinant human TLR5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 661-858 aa of BC109119 Sequence: TKFRGFCFICYKTAQRLVFKDHPQGTEPDMYKYDAYLCFSSKDFTWVQNALLKHLDTQYSDQNRFNLCFEERDFVPGENRIANIQDAIWNSRKIVCLVSRHFLRDGWCLEAFSYAQGRCLSDLNSALIMVVVGSLSQYQLMKHQSIRGFVQKQQYLRWPEDLQDVGWFLHKLSQQILKKEKEKKKDNNIPLQTVATIS Predict reactive species\nFull Name: toll-like receptor 5\nCalculated Molecular Weight: 858 aa, 98 kDa\nObserved Molecular Weight: 100 kDa\nGenBank Accession Number: BC109119\nGene Symbol: TLR5\nGene ID (NCBI): 7100\nRRID: AB_2881931\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: O60602\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888078213289,"sku":"66570-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-66570-1-ig","provider":"Iright","version":"1.0","type":"link"}