{"product_id":"proteintech-66581-1-ig","title":"Proteintech, 66581-1-Ig, STT3A Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe STT3A (66581-1-Ig) by Proteintech is a Monoclonal antibody targeting STT3A in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n66581-1-Ig targets STT3A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: THP-1 cells, K-562 cells,  HL-60 cells,  HEK-293 cells,  RAW 264.7 cells\nPositive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSTT3A, also named as Integral membrane protein 1, belongs to the STT3 family. STT3A is expressed at high levels in placenta, liver, muscle and pancreas, and at very low levels in brain, lung and kidney. STT3A is a catalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser\/Thr consensus motif in nascent polypeptide chains. STT3A is present in the majority of OST complexes and mediates cotranslational N-glycosylation of most sites on target proteins, while STT3B-containing complexes are required for efficient post-translational glycosylation and mediate glycosylation of sites that have been skipped by STT3A. There are two isoforms of STT3A with molecular weight of 81 and 70 kDa. 66581-1-Ig antibody detects a protein around 65-70 kDa in SDS-PAGE which is similar to papers published. (PMID: 32005703, 25135935)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag27072 Product name: Recombinant human STT3A protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 475-705 aa of BC020965 Sequence: STWVTSEAYSSPSIVLSARGGDGSRIIFDDFREAYYWLRHNTPEDAKVMSWWDYGYQITAMANRTILVDNNTWNNTHISRVGQAMASTEEKAYEIMRELDVSYVLVIFGGLTGYSSDDINKFLWMVRIGGSTDTGKHIKENDYYTPTGEFRVDREGSPVLLNCLMYKMCYYRFGQVYTEAKRPPGFDRVRNAEIGNKDFELDVLEEAYTTEHWLVRIYKVKDLDNRGLSRT Predict reactive species\nFull Name: STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)\nCalculated Molecular Weight: 705 aa, 81 kDa\nObserved Molecular Weight: 65 kDa\nGenBank Accession Number: BC020965\nGene Symbol: STT3A\nGene ID (NCBI): 3703\nRRID: AB_2881941\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: P46977\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888017100969,"sku":"66581-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-66581-1-ig","provider":"Iright","version":"1.0","type":"link"}