{"product_id":"proteintech-66755-1-ig","title":"Proteintech, 66755-1-Ig, TRAF4 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe TRAF4 (66755-1-Ig) by Proteintech is a Monoclonal antibody targeting TRAF4 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, pig samples\n66755-1-Ig targets TRAF4 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, pig samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Jurkat cells, HeLa cells,  THP-1 cells,  U-937 cells,  NCCIT cells,  human brain tissue,  pig brain tissue,  HEK-293 cells,  Ramos cells,  Daudi cells,  Raji cells,  pig spleen tissue\nPositive IHC detected in: human urothelial carcinoma tissue, human lung cancer tissue,  human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunohistochemistry (IHC): IHC : 1:200-1:800\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe tumor necrosis factor receptor-associated factor (TRAF) family, consisting of seven members (TRAF1-7), is a group of signaling adaptors which participate in various signaling pathways through binding of the tumor necrosis factor receptor superfamily. As one of the important member of TRAF family proteins, TRAF4 was initially isolated from breast carcinomas and identified as the first member of the TRAF family to be up-regulated in human carcinomas. TRAF4 is required during embryogenesis in key biological processes including the formation of the trachea, the development of the axial skeleton, and the closure of the neural tube. TRAF4 deficiency leads to severe developmental alterations.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, pig\nCited Reactivity: human, mouse\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag27221 Product name: Recombinant human TRAF4 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 35-307 aa of BC001769 Sequence: GHRFCDTCLQEFLSEGVFKCPEDQLPLDYAKIYPDPELEVQVLGLPIRCIHSEEGCRWSGPLRHLQGHLNTCSFNVIPCPNRCPMKLSRRDLPAHLQHDCPKRRLKCEFCGCDFSGEAYESHEGMCPQESVYCENKCGARMMRRLLAQHATSECPKRTQPCTYCTKEFVFDTIQSHQYQCPRLPVACPNQCGVGTVAREDLPGHLKDSCNTALVLCPFKDSGCKHRCPKLAMARHVEESVKPHLAMMCALVSRQRQELQELRRELEELSVGSD Predict reactive species\nFull Name: TNF receptor-associated factor 4\nCalculated Molecular Weight: 54 kDa\nObserved Molecular Weight: 50-54 kDa\nGenBank Accession Number: BC001769\nGene Symbol: TRAF4\nGene ID (NCBI): 9618\nRRID: AB_2882101\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: Q9BUZ4\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888024015017,"sku":"66755-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-66755-1-ig","provider":"Iright","version":"1.0","type":"link"}