{"product_id":"proteintech-66806-1-ig","title":"Proteintech, 66806-1-Ig, EEF1A1\/EEF1A2 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe EEF1A1\/EEF1A2 (66806-1-Ig) by Proteintech is a Monoclonal antibody targeting EEF1A1\/EEF1A2 in WB, IHC, ELISA applications with reactivity to human, mouse, rat, pig samples\n66806-1-Ig targets EEF1A1\/EEF1A2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, MCF-7 cells,  mouse brain tissue,  pig brain tissue,  rat brain tissue,  HEK-293 cells,  Jurkat cells,  HSC-T6 cells,  NIH\/3T3 cells,  4T1 cells\nPositive IHC detected in: rat brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\neEF1A2 is one of two isoforms (eEF1A1 and eEF1A2) of eukaryotic elongation factor 1 alpha (eEF1A or eEF1α). The two isoforms share more than 90% sequence identity and have the similar function that being a protein translation factor involved in protein synthesis. Addition, eEF1A2 plays an important role in cell cycle regulation, heat-shock response, aging, posttranslational modifications, and signal transduction (PMID: 25905039). eEF1A2 is expressed in heart, brain tissue, diaphragm and skeletal muscle while eEF1A1 is expressed ubiquitously (PMID:14588074). And the eEF1A2 protein stimulates the phospholipid signaling and activates the Akt-dependent cell migration and actin remodeling that ultimately favors tumorigenesis. It is reported that eEF1A2 was related with kinds of cancers such as ovarian cancer, prostate cancer, pancreatic cancer, breast cancer and lung cancer (PMID:14588074; 24853801; 25744894; 25905039). An 48 kDa band has also been reported(PMID: 24285179).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat, pig\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag6875 Product name: Recombinant human EEF1A2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 109-463 aa of BC000432 Sequence: ADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPAYSEKRYDEIVKEVSAYIKKIGYNPATVPFVPISGWHGDNMLEPSPNMPWFKGWKVERKEGNASGVSLLEALDTILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGILRPGMVVTFAPVNITTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDIRRGNVCGDSKSDPPQEAAQFTSQVIILNHPGQISAGYSPVIDCHTAHIACKFAELKEKIDRRSGKKLEDNPKSLKSGDAAIVEMVPGKPMCVESFSQYPPLGRFAVRDMRQTVAVGVIKNVEKKSGGAGKVTKSAQKAQKAGK Predict reactive species\nFull Name: eukaryotic translation elongation factor 1 alpha 2\nCalculated Molecular Weight: 50 kDa\nObserved Molecular Weight: 48-50 kDa\nGenBank Accession Number: BC000432\nGene Symbol: EEF1A2\nGene ID (NCBI): 1917\nRRID: AB_2882149\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: Q05639\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888273707177,"sku":"66806-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-66806-1-ig","provider":"Iright","version":"1.0","type":"link"}