{"product_id":"proteintech-66854-1-ig","title":"Proteintech, 66854-1-Ig, Phospho-RIPK1 (Ser161)  Monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-RIPK1 (Ser161)  (66854-1-Ig) by Proteintech is a Monoclonal antibody targeting Phospho-RIPK1 (Ser161)  in WB, IHC, IF\/ICC, FC (Intra), ELISA applications with reactivity to human samples\n66854-1-Ig targets Phospho-RIPK1 (Ser161)  in WB, IHC, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Jurkat cells, Calyculin A treated HeLa cells,  Raji cells,  THP-1 cells\nPositive IHC detected in: human small intestine tissue, human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: U2OS cells, Calyculin A treated HeLa cells\nPositive FC (Intra) detected in: Calyculin A treated HEK-293T cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000- 1:20000\nImmunohistochemistry (IHC): IHC : 1:250-1:1000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:400-1:1600\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nRIPK1(Receptor-interacting serine\/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis and necroptosis and belongs to a novel class of kinases thatfunction in cell survival and cell death mechanisms(PMID:22685397 ).It has 2 isoforms produced by alternative splicing.It also can exist as the protein with lower molecular weight about 25kd and 45kd in HAEC and HUVEC(PMID:22685397).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse, rat, pig\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: receptor (TNFRSF)-interacting serine-threonine kinase 1\nCalculated Molecular Weight: 76 kDa\nObserved Molecular Weight: 74 kDa, 45 kDa\nGenBank Accession Number: NM_003804\nGene Symbol: RIPK1\nGene ID (NCBI): 8737\nRRID: AB_2882194\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: Q13546\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887984988329,"sku":"66854-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-66854-1-ig","provider":"Iright","version":"1.0","type":"link"}