{"product_id":"proteintech-66937-1-ig","title":"Proteintech, 66937-1-Ig, UBR5 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe UBR5 (66937-1-Ig) by Proteintech is a Monoclonal antibody targeting UBR5 in WB, IHC, ELISA applications with reactivity to Human, mouse, rat samples\n66937-1-Ig targets UBR5 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: MCF-7 cells, HeLa cells,  PC-12 cells,  HEK-293 cells,  Jurkat cells,  Neuro-2a cells\nPositive IHC detected in: mouse testis tissue, mouse brain tissue,  rat testis tissue,  rat brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nUBR5, also named as ubiquitin protein ligase E3 component n-reconin5, is a HECT domain-containing protein as a myocardin-binding protein. UBR5 is highly conserved in metazoans, has unique structural features, and has been implicated in regulation of DNA damage response, metabolism, transcription, and apoptosis. Hence, UBR5 is a key regulator of cell signaling relevant to broad areas of cancer biology (PMID: 26464214). The human UBR5 gene has 59 exons, encoding an approximately 10kb mRNA and \u0026gt;300 kDa protein with widespread expression in various cell types.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: Human, mouse, rat\nCited Reactivity: human, mouse, ae. aegypti\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag18753 Product name: Recombinant human UBR5 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 2450-2799 aa of BC137234 Sequence: IAHGRENGADSILDLGLVDSSEKVQQENRKRHGSSRSVVDMDLDDTDDGDDNAPLFYQPGKRGFYTPRPGKNTEARLNCFRNIGRILGLCLLQNELCPITLNRHVIKVLLGRKVNWHDFAFFDPVMYESLRQLILASQSSDADAVFSAMDLAFAIDLCKEEGGGQVELIPNGVNIPVTPQNVYEYVRKYAEHRMLVVAEQPLHAMRKGLLDVLPKNSLEDLTAEDFRLLVNGCGEVNVQMLISFTSFNDESGENAEKLLQFKRWFWSIVEKMSMTERQDLVYFWTSSPSLPASEEGFQPMPSITIRPPDDQHLPTANTCISRLYVPLYSSKQILKQKLLLAIKTKNFGFV Predict reactive species\nFull Name: ubiquitin protein ligase E3 component n-recognin 5\nCalculated Molecular Weight: 2799 aa, 309 kDa\nObserved Molecular Weight: 309 kDa\nGenBank Accession Number: BC137234\nGene Symbol: UBR5\nGene ID (NCBI): 51366\nRRID: AB_2882261\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: O95071\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887999504553,"sku":"66937-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-66937-1-ig","provider":"Iright","version":"1.0","type":"link"}