{"product_id":"proteintech-67022-1-ig","title":"Proteintech, 67022-1-Ig, TIMELESS Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe TIMELESS (67022-1-Ig) by Proteintech is a Monoclonal antibody targeting TIMELESS in WB, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples\n67022-1-Ig targets TIMELESS in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, U2OS cells,  A549 cells,  JAR cells,  Jurkat cells,  HSC-T6 cells,  NIH\/3T3 cells,  HEK-293 cells,  K-562 cells\nPositive FC (Intra) detected in: HEK-293 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nTIMELESS1 (TIM1) gene encodes protein timeless homolog belonging to timeless family.  TIM1 is localized in nucleus and regulated by cell cycle. It expresses high levels in S, G2 and M phases, and low in G0 and G1 phases. TIM1 is involved in the circadian rhythm autoregulatory loop and is a crucial component of the circadian core oscillator, which includes the CRY proteins, CLOCK, NPAS2, etc. It is also involved in cell survival after DNA damage or replication stress and reported to play important role in epithelial cell morphogenesis and formation of branching tubules.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, canine\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag6283 Product name: Recombinant human TIMELESS protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 869-1208 aa of BC050557 Sequence: QMGLADSVKDFQRKGTHIVLWTGDQELELQRLFEEFRDSDDVLGHIMKNITAKRSRARIVDKLLALGLVAERRELYKKRQKKLASSILPNGAESLKDFCQEDLEEEENLPEEDSEEEEEGGSEAEQVQGSLVLSNENLGQSLHQEGFSILLLWLQNCLIRAADDREEDGCSQAVPLVPLTEENEEAMENEQFQQLLRKLGVRPPASGQETFWRIPAKLSPTQLRRAAASLSQPEEEQKLQPELQPKVPGEQGSDEEHCKEHRAQALRALLLAHKKKAGLASPEEEDAVGKEPLKAAPKKRQLLDSDEEQEEDEGRNRAPELGAPGIQKKKRYQIEDDEDD Predict reactive species\nFull Name: timeless homolog (Drosophila)\nCalculated Molecular Weight: 139 kDa\nObserved Molecular Weight: 140 kDa\nGenBank Accession Number: BC050557\nGene Symbol: TIMELESS\nGene ID (NCBI): 8914\nRRID: AB_2882337\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: Q9UNS1\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888078082217,"sku":"67022-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-67022-1-ig","provider":"Iright","version":"1.0","type":"link"}