{"product_id":"proteintech-67027-1-ig","title":"Proteintech, 67027-1-Ig, PRC1 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe PRC1 (67027-1-Ig) by Proteintech is a Monoclonal antibody targeting PRC1 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n67027-1-Ig targets PRC1 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, MCF-7 cells,  HEK-293 cells,  A549 cells,  HepG2 cells,  HSC-T6 cells,  4T1 cells\nPositive IHC detected in: human breast cancer tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunohistochemistry (IHC): IHC : 1:200-1:1000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:400-1:1600\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPRC1 (protein regulator of cytokinesis 1) is a microtubule-bundling protein that is involved in cytokinesis. Depletion of PRC1 leads to failed cytokinesis. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a substrate of CDK1 and it was assumed that CDK1 phosphorylation blocks its activity during metaphase. Three isoforms of PRC1 exist due to the alternative splicing, with predictive molecular weight of 71.6 kDa, 70.2 kDa and 66.2 kDa, respectively. The dual bands between 66-72 kDa detected by this antibody may represent different forms of PRC1.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag5907 Product name: Recombinant human PRC1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 154-446 aa of BC003138 Sequence: NQFRQHVTTLRETKASRREEFVSIKRQIILCMEELDHTPDTSFERDVVCEDEDAFCLSLENIATLQKLLRQLEMQKSQNEAVCEGLRTQIRELWDRLQIPEEEREAVATIMSGSKAKVRKALQLEVDRLEELKMQNMKKVIEAIRVELVQYWDQCFYSQEQRQAFAPFCAEDYTESLLQLHDAEIVRLKNYYEVHKELFEGVQKWEETWRLFLEFERKASDPNRFTNRGGNLLKEEKQRAKLQKMLPKLEEELKARIELWEQEHSKAFMVNGQKFMEYVAEQWEMHRLEKERA Predict reactive species\nFull Name: protein regulator of cytokinesis 1\nCalculated Molecular Weight: 72 kDa\nObserved Molecular Weight: 66-72 kDa\nGenBank Accession Number: BC003138\nGene Symbol: PRC1\nGene ID (NCBI): 9055\nRRID: AB_2882342\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: O43663\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888313618601,"sku":"67027-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-67027-1-ig","provider":"Iright","version":"1.0","type":"link"}