{"product_id":"proteintech-67033-1-ig","title":"Proteintech, 67033-1-Ig, CYP1B1 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe CYP1B1 (67033-1-Ig) by Proteintech is a Monoclonal antibody targeting CYP1B1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat, pig samples\n67033-1-Ig targets CYP1B1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, PC-3 cells,  rat brain tissue,  mouse brain tissue,  pig brain tissue,  T-47D cells,  MCF-7 cells\nPositive IHC detected in: mouse heart tissue, human rectal cancer tissue,  mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:3000-1:10000\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCytochrome P450 CYP1B1 is a recently cloned dioxin-inducible form of the cytochrome P450 family of xenobiotic metabolizing enzymes. CYP1B1 was found to be expressed at a high frequency in a wide range of human cancers of different histogenetic types, including cancers of the breast, colon, lung, esophagus, skin, lymph node, brain, and testis. There was no detectable immunostaining for CYP1B1 in normal tissues.(PMID:9230218). CYP1B1 is a 60 kDa protein that it also can be detected a band of 51 kDa or 57 kDa through the western blot(PMID:9525272; PMID: 9525272).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat, pig\nCited Reactivity: human, mouse\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag13380 Product name: Recombinant human CYP1B1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-259 aa of BC012049 Sequence: MGTSLSPNDPWPLNPLSIQQTTLLLLLSVLATVHVGQRLLRQRRRQLRSAPPGPFAWPLIGNAAAVGQAAHLSFARLARRYGDVFQIRLGSCPIVVLNGERAIHQALVQQGSAFADRPAFASFRVVSGGRSMAFGHYSEHWKVQRRAAHSMMRNFFTRQPRSRQVLEGHVLSEARELVALLVRGSADGAFLDPRPLTVVAVANVMSAVCFGCRYSHDDPEFRELLSHNEEFGRTVGAGSLVDVMPWLQYFPNPVRTVFR Predict reactive species\nFull Name: cytochrome P450, family 1, subfamily B, polypeptide 1\nCalculated Molecular Weight: 61 kDa\nObserved Molecular Weight: 52 kDa\nGenBank Accession Number: BC012049\nGene Symbol: CYP1B1\nGene ID (NCBI): 1545\nRRID: AB_2882348\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: Q16678\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888039448745,"sku":"67033-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-67033-1-ig","provider":"Iright","version":"1.0","type":"link"}