{"product_id":"proteintech-67103-1-ig","title":"Proteintech, 67103-1-Ig, MCM4 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe MCM4 (67103-1-Ig) by Proteintech is a Monoclonal antibody targeting MCM4 in WB, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n67103-1-Ig targets MCM4 in WB, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HSC-T6 cells,  NIH\/3T3 cells,  HEK-293 cells,  A431 cells,  HL-60 cells,  Jurkat cells\nPositive IF\/ICC detected in: U2OS cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:20000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:400-1:1600\n\u003cb\u003eBackground Information\u003c\/b\u003e\nDNA replication licensing factor MCM4 (MCM4), also named Cdc21, acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of \ntwo neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box \nof the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Mouse \/ IgG2b\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag28642 Product name: Recombinant human MCM4 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 515-859 aa of BC031061 Sequence: SKSQLLQYVYNLVPRGQYTSGKGSSAVGLTAYVMKDPETRQLVLQTGALVLSDNGICCIDEFDKMNESTRSVLHEVMEQQTLSIAKAGIICQLNARTSVLAAANPIESQWNPKKTTIENIQLPHTLLSRFDLIFLMLDPQDEAYDRRLAHHLVALYYQSEEQAEEELLDMAVLKDYIAYAHSTIMPRLSEEASQALIEAYVDMRKIGSSRGMVSAYPRQLESLIRLAEAHAKVRLSNKVEAIDVEEAKRLHREALKQSATDPRTGIVDISILTTGMSATSRKRKEELAEALKKLILSKGKTPALKYQQLFEDIRGQSDIAITKDMFEEALRALADDDFLTVTGKT Predict reactive species\nFull Name: minichromosome maintenance complex component 4\nCalculated Molecular Weight: 863 aa, 97 kDa\nObserved Molecular Weight: 97 kDa\nGenBank Accession Number: BC031061\nGene Symbol: MCM4\nGene ID (NCBI): 4173\nRRID: AB_2882408\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P33991\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888067268777,"sku":"67103-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-67103-1-ig","provider":"Iright","version":"1.0","type":"link"}