{"product_id":"proteintech-67766-1-ig","title":"Proteintech, 67766-1-Ig, gp130\/IL6ST Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe gp130\/IL6ST (67766-1-Ig) by Proteintech is a Monoclonal antibody targeting gp130\/IL6ST in WB, IHC, ELISA applications with reactivity to human samples\n67766-1-Ig targets gp130\/IL6ST in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HepG2 cells, NCCIT cells,  PC-3 cells,  DU 145 cells\nPositive IHC detected in: human placenta tissue, human colon cancer tissue,  human liver cancer tissue,  human lung cancer tissue,  human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nGlycoprotein 130 (gp130, also known as IL6ST, CD130 or IL6-beta) is a ubiquitously expressed, signal-transducing receptor that serves as the signal transduction unit for IL-6 family of cytokines, including IL-6, IL-11, IL-27, leukemia inhibitory factor (LIF), OSM, ciliary neurotrophic factor (CNTF), cardiotrophin 1 (CT-1), and cardiotrophin-like cytokine (CLC) (PMID: 9143707; 20610800). These cytokines signal through the gp130\/Jak\/STAT pathway (PMID: 9716487). Binding of IL-6 to IL-6R induces gp130 homodimerization and formation of a high-affinity receptor complex, which activates Jaks. That causes phosphorylation of gp130 tyrosine residues which in turn activates STAT3. gp130 is a type I transmembrane protein, and can also exist as a soluble form (sgp130). sgp130 binds to sIL-6R\/IL-6 complexes and prevents their interactions with membrane-anchored gp130 on target cells (PMID: 9815830).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag28808 Product name: Recombinant human IL-6ST protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 124-369 aa of NM_002184 Sequence: MLPPEKPKNLSCIVNEGKKMRCEWDGGRETHLETNFTLKSEWATHKFADCKAKRDTPTSCTVDYSTVYFVNIEVWVEAENALGKVTSDHINFDPVYKVKPNPPHNLSVINSEELSSILKLTWTNPSIKSVIILKYNIQYRTKDASTWSQIPPEDTASTRSSFTVQDLKPFTEYVFRIRCMKEDGKGYWSDWSEEASGITYEDRPSKAPSFWYKIDPSHTQGYRTVQLVWKTLPPFEANGKILDYEVT Predict reactive species\nFull Name: interleukin 6 signal transducer (gp130, oncostatin M receptor)\nCalculated Molecular Weight: 104 kDa\nObserved Molecular Weight: 130-140 kDa\nGenBank Accession Number: NM_002184\nGene Symbol: IL6ST\nGene ID (NCBI): 3572\nRRID: AB_2918533\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: P40189\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888015495337,"sku":"67766-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-67766-1-ig","provider":"Iright","version":"1.0","type":"link"}