{"product_id":"proteintech-68055-1-ig","title":"Proteintech, 68055-1-Ig, m6A Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe m6A (68055-1-Ig) by Proteintech is a Monoclonal antibody targeting m6A in IHC, RIP, Dot Blot, ELISA applications with reactivity to chemical compound, m6a samples\n68055-1-Ig targets m6A in WB, IHC, IF, IP, ChIP, RIP, Dot Blot, ELISA applications and shows reactivity with chemical compound, m6a samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive RIP detected in: RNA\nPositive IHC detected in: mouse testis tissue, human lung cancer tissue,  human breast cancer tissue,  human colon cancer tissue,  rat lymph nodeNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive ELISA detected in: m6A\nPositive Dot Blot detected in: RNA\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nRIP: RIP : 5-10μg antibody\/mg lysate\nImmunohistochemistry (IHC): IHC : 1:2000-1:8000\nEnzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:2000-1:20000\nDOT BLOT: DOT BLOT : 1:1000-1:4000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nm6A (N6-methyladenosine) is the most abundant internal modification in mammalian mRNA. This modification is installed by the m6A methyltransferases or termed \"writers\"such as METTL3 and METTL14, and can be reversed by demethylases that serve as \"erasers\" such as FTO and ALKBH5. The stability of m6A-modified mRNA is regulated by m6A reader protein YTHDFs, which recognizes m6A and reduces the stability of target transcripts. m6A modification and its regulatory proteins play critical roles in cancer pathogenesis and progression. m6A modification is also invovled in viruses life cycles, suggesting that drugs targets to m6A pathway could be used for antiviral thereapy.Protocol for Dot Blot:https:\/\/www.ptglab.com\/protocol\/68055-1-IgDotBlot.pdf\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: chemical compound, m6a\nCited Reactivity: human, mouse, rat, pig, monkey\nHost \/ Isotype: Mouse \/ IgG3\nClass: Monoclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: m6A\nGenBank Accession Number: m6A\nRRID: AB_2918796\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887972602025,"sku":"68055-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-68055-1-ig","provider":"Iright","version":"1.0","type":"link"}