{"product_id":"proteintech-68194-1-ig","title":"Proteintech, 68194-1-Ig, NNT Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe NNT (68194-1-Ig) by Proteintech is a Monoclonal antibody targeting NNT in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to Human, Mouse, Rat, Rabbit, Pig samples\n68194-1-Ig targets NNT in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: pig heart tissue, LNCaP cells,  HeLa cells,  Jurkat cells,  rabbit heart tissue,  rat heart tissue,  mouse heart tissue,  rat liver tissue,  mouse liver tissue\nPositive IP detected in: HepG2 cells\nPositive IHC detected in: human liver tissue, mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:400-1:1600\n\u003cb\u003eBackground Information\u003c\/b\u003e\nNNT(nicotinamide nucleotide transhydrogenase) is a transmembrane protein and functions as a proton pumping transhydrogenase. The protein is present in both prokaryotes and eukaryotes and is located in the inner membrane of mitochondria. In prokaryotic cells, the enzyme is composed of α and β subunits of 54 and 48 kDa, respectively. In eukaryotic cells, the enzyme is usually composed of a single peptide of 114 kDa. Although NNT catalyzes the interconversion of NADH and NADPH, the forward reaction using the reducing power of NADH to regenerate NADPH would be favored under conditions of oxidative stress(PMID:16497723). It can exsit as a homodimer(PMID:21882037).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: Human, Mouse, Rat, Rabbit, Pig\nCited Reactivity: mouse, rat\nHost \/ Isotype: Mouse \/ IgG2b\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag4259 Product name: Recombinant human NNT protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 30-236 aa of BC032370 Sequence: MNRSLANVILGGYGTTSTAGGKPMEISGTHTEINLDNAIDMIREANSIIITPGYGLCAAKAQYPIADLVKMLTEQGKKVRFGIHPVAGRMPGQLNVLLAEAGVPYDIVLEMDEINHDFPDTDLVLVIGANDTVNSAAQEDPNSIIAGMPVLEVWKSKQVIVMKRSLGVGYAAVDNPIFYKPNTAMLLGDAKKTCDALQAKVRESYQK Predict reactive species\nFull Name: nicotinamide nucleotide transhydrogenase\nCalculated Molecular Weight: 1085 aa, 114 kDa\nObserved Molecular Weight: 114 kDa\nGenBank Accession Number: BC032370\nGene Symbol: NNT\nGene ID (NCBI): 23530\nRRID: AB_2935283\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q13423\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888069365929,"sku":"68194-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-68194-1-ig","provider":"Iright","version":"1.0","type":"link"}