{"product_id":"proteintech-68259-1-ig","title":"Proteintech, 68259-1-Ig, CHCHD3\/MIC19 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe CHCHD3\/MIC19 (68259-1-Ig) by Proteintech is a Monoclonal antibody targeting CHCHD3\/MIC19 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat, pig, rabbit samples\n68259-1-Ig targets CHCHD3\/MIC19 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: pig heart tissue, A549 cells,  HeLa cells,  rabbit heart tissue,  rat heart tissue,  mouse heart tissue,  rat brain tissue\nPositive IHC detected in: human lung tissue, human lung cancer tissue,  mouse cerebellum tissue,  mouse lung tissue,  rat cerebellum tissue,  rat heart tissue,  rat lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:400-1:1600\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCHCHD3, initially identified as a substrate for cAMP-dependent protein kinase (PKA), is a ubiquitous protein in the mitochondria and plays a prominent role in maintaining cristae integrity and mitochondrial function. In mitochondria, ChChd3 is predominantly localized to the inner membrane (IM), facing toward the intermembrane space (IMS), and is part of the large protein complex now called as MINOS (mitochondrial inner membrane organizing system), or MIB (mitochondrial intermembrane space bridging) CHCHD3 is highly conserved in mammals with human and mouse protein sharing ∼92% sequence similarity.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat, pig, rabbit\nCited Reactivity: rat\nHost \/ Isotype: Mouse \/ IgG2a\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag22628 Product name: Recombinant human CHCHD3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-227 aa of BC014839 Sequence: MGGTTSTRRVTFEADENENITVVKGIRLSENVIDRMKESSPSGSKSQRYSGAYGASVSDEELKRRVAEELALEQAKKESEDQKRLKQAKELDRERAAANEQLTRAILRERICSEEERAKAKHLARQLEEKDRVLKKQDAFYKEQLARLEERSSEFYRVTTEQYQKAAEEVEAKFKRYESHPVCADLQAKILQCYRENTHQTLKCSALATQYMHCVNHAKQSMLEKGG Predict reactive species\nFull Name: coiled-coil-helix-coiled-coil-helix domain containing 3\nCalculated Molecular Weight: 227 aa, 26 kDa\nObserved Molecular Weight: 26 kDa\nGenBank Accession Number: BC014839\nGene Symbol: CHCHD3\nGene ID (NCBI): 54927\nRRID: AB_2935344\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q9NX63\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888058945705,"sku":"68259-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-68259-1-ig","provider":"Iright","version":"1.0","type":"link"}