{"product_id":"proteintech-68301-1-ig","title":"Proteintech, 68301-1-Ig, 5-methylcytosine Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe 5-methylcytosine (68301-1-Ig) by Proteintech is a Monoclonal antibody targeting 5-methylcytosine in IHC, Dot Blot, ELISA applications with reactivity to 5mc, chemical compound, m5c samples\n68301-1-Ig targets 5-methylcytosine in WB, IHC, IF, RIP, Dot Blot, ELISA applications and shows reactivity with 5mc, chemical compound, m5c samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive IHC detected in: mouse testis tissue, rat brain tissue,  rat testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive ELISA detected in: compound\nPositive Dot Blot detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nImmunohistochemistry (IHC): IHC : 1:2500-1:10000\nEnzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:10-1:100\nDOT BLOT: DOT BLOT : 1:2500-1:10000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nIn eukaryotes, 5-methylcytosine in DNA (5mC) and its oxidized derivatives (5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC)) are the most prominent modifications and have been suggested to contribute to epigenetic gene regulation through a variety of different mechanisms. m5C has also been found in mRNA, rRNA and tRNA of representative organisms from all kinds of species. As reversible epigenetic modifications, m5C modifications of RNA affect the fate of the modified RNA molecules and play important roles in various biological processes including RNA stability control, protein synthesis, and transcriptional regulation (PMID: 35365216).\nThis antibody can recognize both 5mC in DNA and m5C in RNA.Protocol for Dot Blot:https:\/\/www.ptglab.com\/protocol\/68301-1-IgDotBlot.pdf\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: 5mc, chemical compound, m5c\nCited Reactivity: human, mouse\nHost \/ Isotype: Mouse \/ IgG2b\nClass: Monoclonal\nType: Antibody\nImmunogen: Compound Predict reactive species\nFull Name: 5-methylcytosine\nRRID: AB_2935380\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887989117097,"sku":"68301-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-68301-1-ig","provider":"Iright","version":"1.0","type":"link"}