{"product_id":"proteintech-68380-1-ig","title":"Proteintech, 68380-1-Ig, Tuberin\/TSC2 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe Tuberin\/TSC2 (68380-1-Ig) by Proteintech is a Monoclonal antibody targeting Tuberin\/TSC2 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n68380-1-Ig targets Tuberin\/TSC2 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A549 cells, MCF-7 cells,  PC-3 cells,  HSC-T6 cells,  NIH\/3T3 cells,  HeLa cells,  U2OS cells,  LNCaP cells,  HEK-293 cells,  Jurkat cells,  K-562 cells\nPositive IHC detected in: rat brain tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HEK-293 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:400-1:1600\n\u003cb\u003eBackground Information\u003c\/b\u003e\nTSC2, also named Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: tuberous sclerosis 2\nCalculated Molecular Weight: 1807 aa, 201 kDa\nObserved Molecular Weight: 200 kDa\nGenBank Accession Number: BC150300\nGene Symbol: TSC2\nGene ID (NCBI): 7249\nRRID: AB_3085098\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: P49815\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888078999721,"sku":"68380-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-68380-1-ig","provider":"Iright","version":"1.0","type":"link"}