{"product_id":"proteintech-68420-1-ig","title":"Proteintech, 68420-1-Ig, Nesprin 3 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe Nesprin 3 (68420-1-Ig) by Proteintech is a Monoclonal antibody targeting Nesprin 3 in WB, IF\/ICC, IF-P, ELISA applications with reactivity to human, mouse, rat samples\n68420-1-Ig targets Nesprin 3 in WB, IF\/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HepG2 cells, HEK-293 cells,  LNCaP cells,  HeLa cells,  Jurkat cells,  MOLT-4 cells,  C2C12 cells,  PC-12 cells,  rat testis tissue\nPositive IF-P detected in: mouse testis tissue\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunofluorescence (IF)-P: IF-P : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:400-1:1600\n\u003cb\u003eBackground Information\u003c\/b\u003e\nNesprin 3 also known as C14orf49, SYNE3, belongs to the Nesprin family. Nesprin-3 is a type II transmembrane protein of the outer nuclear membrane and contains a C-terminal KASH domain and a series of spectrin repeats(PMID: 16330710). Nesprin-3, a protein that links intermediate filaments to the nucleus, regulates endothelial cell morphology, perinuclear cytoskeletal organization, and flow-induced cell polarization and migration. Nesprin-3 is robustly expressed in human aortic endothelial cells and localizes to the nuclear envelope(PMID: 21937718).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag26012 Product name: Recombinant human C14orf49 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-300 aa of BC146604 Sequence: MTQQPQDDFDRSVEDAQAWMKAVQDQLQVNDNTQGPRAALEARLWETEKICQLEPEGRVRVDLVLRMAEALLACCPGDQKPGILARLKDIKAQWEETVTYMTHCHSRIEWVWLHWSEYLLARDEFYRWFQKMMVTLEPHIELQLGLKEKQWQLSHAQVLLHNVDNQAVLLDRLLEEAASLFNRIGDPSVDEDAQKRMKAEYDAVKAKAQKRVDLLEQVAREHEEYQAGVDEFQLWLKAVVEKVNGCLGRNCKLPITQRLSTLQDIAKDFPRGEESLETLEEQSAGVIRNTSPLGAEKITG Predict reactive species\nFull Name: chromosome 14 open reading frame 49\nObserved Molecular Weight: 110 kDa\nGenBank Accession Number: BC146604\nGene Symbol: Nesprin 3\nGene ID (NCBI): 161176\nRRID: AB_3085135\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: Q6ZMZ3\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888302641321,"sku":"68420-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-68420-1-ig","provider":"Iright","version":"1.0","type":"link"}