{"product_id":"proteintech-68578-1-ig","title":"Proteintech, 68578-1-Ig, Pseudouridine Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe Pseudouridine (68578-1-Ig) by Proteintech is a Monoclonal antibody targeting Pseudouridine in Dot Blot, ELISA applications with reactivity to chemical compound samples\n68578-1-Ig targets Pseudouridine in Dot Blot, ELISA applications and shows reactivity with chemical compound samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive ELISA detected in: Pseudouridine\nPositive Dot Blot detected in: RNA\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nEnzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:2000-1:20000\nDOT BLOT: DOT BLOT : 1:1000-1:4000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPseudouridine is an isomer of the nucleoside uridine in which the uracil is attached via a carbon-carbon instead of a nitrogen-carbon glycosidic bond. Pseudouridine is the most abundant RNA modification in cellular RNA (PMID: 29104216). After transcription and following synthesis, RNA can be modified with over 100 chemically distinct modifications. These can potentially regulate RNA expression post-transcriptionally, in addition to the four standard nucleotides and play a variety of roles in the cell including translation, localization and stabilization of RNA. Pseudouridine in rRNA and tRNA has been shown to fine-tune and stabilize the regional structure and help maintain their functions in mRNA decoding, ribosome assembly, processing and translation (PMID: 10902565,  PMID: 23391857,  PMID: 28045575). Pseudouridine in snRNA has been shown to enhance spliceosomal RNA-pre-mRNA interaction to facilitate splicing regulation (PMID 27268497).Protocol for Dot Blot:https:\/\/www.ptglab.com\/protocol\/68578-1-IgDotBlot.pdf\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: chemical compound\nCited Reactivity: human\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: PTG Predict reactive species\nFull Name: Pseudouridine\nRRID: AB_3085278\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888048459945,"sku":"68578-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-68578-1-ig","provider":"Iright","version":"1.0","type":"link"}