{"product_id":"proteintech-68787-3-ig","title":"Proteintech, 68787-3-Ig, RNASEH1 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe RNASEH1 (68787-3-Ig) by Proteintech is a Monoclonal antibody targeting RNASEH1 in WB, ELISA applications with reactivity to human, mouse, rat samples\n68787-3-Ig targets RNASEH1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: SK-N-SH cells, 4T1 cells,  A549 cells,  HeLa cells,  HEK-293 cells,  Jurkat cells,  K-562 cells,  HSC-T6 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nRibonucleases H are enzymes that cleave the RNA of RNA\/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts [PMID:12667461]. Eukaryotic RNase H1 has acquired a Hybrid Binding Domain that confers processivity and affinity for the substrate. Because of its essential role and its similarities in structure and enzymatic mechanism to the RNase H of HIV reverse transcriptase, RNASEH1 has been implicated in mitochondrial DNA replication during mouse development [PMID:19228196].\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag33948 Product name: Recombinant human RNASEH1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-286 aa of BC002973 Sequence: MSWLLFLAHRVALAALPCRRGSRGFGMFYAVRRGRKTGVFLTWNECRAQVDRFPAARFKKFATEDEAWAFVRKSASPEVSEGHENQHGQESEAKASKRLREPLDGDGHESAEPYAKHMKPSVEPAPPVSRDTFSYMGDFVVVYTDGCCSSNGRRRPRAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIEQAKTQNINKLVLYTDSMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFVALERLTQGMDIQWMHVPGHSGFIGNEEADRLAREGAKQSED Predict reactive species\nFull Name: ribonuclease H1\nCalculated Molecular Weight: 32 kDa\nObserved Molecular Weight: 32 kDa\nGenBank Accession Number: BC002973\nGene Symbol: RNASEH1\nGene ID (NCBI): 246243\nRRID: AB_3670429\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: O60930\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888319221929,"sku":"68787-3-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-68787-3-ig","provider":"Iright","version":"1.0","type":"link"}