{"product_id":"proteintech-68999-1-ig","title":"Proteintech, 68999-1-Ig, Phospho-IkB Alpha (Ser32\/36) Monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-IkB Alpha (Ser32\/36) (68999-1-Ig) by Proteintech is a Monoclonal antibody targeting Phospho-IkB Alpha (Ser32\/36) in WB, ELISA applications with reactivity to human samples\n68999-1-Ig targets Phospho-IkB Alpha (Ser32\/36) in WB, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: PC-3 cells, MCF-7 cells,  Calyculin A treated MCF-7 cells,  Calyculin A treated PC cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nNuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI).  NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime. The antibody also detects the phosphorylated form of the NFKBIA (IkB Alpha).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: mouse\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha\nCalculated Molecular Weight: 36 kDa\nObserved Molecular Weight: 36-39 kDa\nGenBank Accession Number: BC004983\nGene Symbol: IkB Alpha\nGene ID (NCBI): 4792\nRRID: AB_3670459\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: P25963\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888070840489,"sku":"68999-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-68999-1-ig","provider":"Iright","version":"1.0","type":"link"}