{"product_id":"proteintech-80007-1-rr","title":"Proteintech, 80007-1-RR, Phospho-TDP43 (Ser409\/410) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-TDP43 (Ser409\/410) (80007-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-TDP43 (Ser409\/410) in WB, IHC, ELISA applications with reactivity to human, mouse samples\n80007-1-RR targets Phospho-TDP43 (Ser409\/410) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, Calyculin A treated cells\nPositive IHC detected in: frontal cortex of FTLD-TDP type B case, human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nTransactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 80007-1-RR is a recombinant rabbit monoclonal antibody recognizing TDP-43 only when phosphorylated at 409\/410. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse, zebrafish\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: TAR DNA binding protein\nCalculated Molecular Weight: 43 kDa\nObserved Molecular Weight: 45-50 kDa\nGenBank Accession Number: NM_007375\nGene Symbol: TDP-43\nGene ID (NCBI): 23435\nRRID: AB_2882937\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q13148\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888342782121,"sku":"80007-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-80007-1-rr","provider":"Iright","version":"1.0","type":"link"}