{"product_id":"proteintech-80059-1-rr","title":"Proteintech, 80059-1-RR, Phospho-EIF4B (Ser406) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-EIF4B (Ser406) (80059-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-EIF4B (Ser406) in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n80059-1-RR targets Phospho-EIF4B (Ser406) in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293T cells, HeLa cells,  Calyculin A treated HEK-293T cells,  PC-3 cells,  Calyculin A treated PC-3 cells,  Calyculin A treated Hela cells,  HSC-T6 cells,  NIH\/3T3 cells,  4T1 cells\nPositive IHC detected in: Jurkat cells, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunohistochemistry (IHC): IHC : 1:2000-1:8000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nEIF4B is one of the mammalian eukaryotic initiation factors (eIF) that are required for the ATP-dependent binding of mRNA to the 40 S ribosomal subunit, and the other eIF proteins are EIF4A, EIF4F. eIF4B is involved in translation of numerous proliferative or anti-apoptotic mRNAs with highly structured 5'UTR and subsequently affect cell growth and survival. It was reported that false expression and phosphorylation levels of eIF4B are involved in several tumors including breast cancer, cell lymphoblastic leukemia and diffuse large B-cell lymphoma (PMID: 26848623).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: eukaryotic translation initiation factor 4B\nCalculated Molecular Weight: 611 aa, 69 kDa\nObserved Molecular Weight: 70-80 kDa\nGenBank Accession Number: BC073154\nGene Symbol: EIF4B\nGene ID (NCBI): 1975\nRRID: AB_2918859\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P23588\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888377286825,"sku":"80059-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-80059-1-rr","provider":"Iright","version":"1.0","type":"link"}