{"product_id":"proteintech-80077-1-rr","title":"Proteintech, 80077-1-RR, P53 Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe P53 (80077-1-RR) by Proteintech is a Recombinant antibody targeting P53 in WB, IHC-Autostainer, IF\/ICC, FC (Intra), ELISA applications with reactivity to human, rat, zebrafish samples\n80077-1-RR targets P53 in WB, IHC-Autostainer, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat, zebrafish samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: COLO 320 cells, A431 cells,  HSC-T6 cells,  HT-29 cells,  ROS1728 cells,  SW480 cells,  zebrafish tissue\nPositive IHC-Autostainer detected in: human colon cancer tissue, human ovarian cancer\nPositive IF\/ICC detected in: HepG2 cells, A431 cells\nPositive FC (Intra) detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC)-AUTOSTAINER: IHC-AUTOSTAINER : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nTP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. \nTP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, rat, zebrafish\nCited Reactivity: human, mouse, zebrafish\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: tumor protein p53\nCalculated Molecular Weight: 44 kDa\nObserved Molecular Weight: 53 kDa\nGenBank Accession Number: BC003596\nGene Symbol: P53\nGene ID (NCBI): 7157\nRRID: AB_2918862\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P04637\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888346484905,"sku":"80077-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-80077-1-rr","provider":"Iright","version":"1.0","type":"link"}