{"product_id":"proteintech-80108-1-rr","title":"Proteintech, 80108-1-RR, Phospho-RPS6KA1 (Ser380) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-RPS6KA1 (Ser380) (80108-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-RPS6KA1 (Ser380) in WB, IHC, IF\/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples\n80108-1-RR targets Phospho-RPS6KA1 (Ser380) in WB, IHC, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: PDGF treated NIH\/3T3 cells, HeLa cells,  Jurkat cells,  Calyculin A treated HeLa cells,  Calyculin A treated Jurkat cells\nPositive IHC detected in: human malignant melanoma tissue, human placenta tissue,  Jurkat cells,  mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\nPositive FC (Intra) detected in: TPA treated Jurkat cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:5000\nImmunohistochemistry (IHC): IHC : 1:1000-1:4000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:250-1:1000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine\/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1\/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1\/ER81 and NR4A1\/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation. It has been reported that the phosphorylation of RPS6KA1 at Ser380 is involved in autophosphorylation by activation of the C-terminal kinase domain. (PMID: 16458888, 30813401, 17259979, 10679322)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: ribosomal protein S6 kinase, 90kDa, polypeptide 1\nCalculated Molecular Weight: 735 aa, 83 kDa\nObserved Molecular Weight: 83-90 kDa\nGenBank Accession Number: BC014966\nGene Symbol: RPS6KA1\nGene ID (NCBI): 6195\nRRID: AB_2918865\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q15418\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888345796777,"sku":"80108-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-80108-1-rr","provider":"Iright","version":"1.0","type":"link"}