{"product_id":"proteintech-80138-2-rr","title":"Proteintech, 80138-2-RR, Phospho-STAT5A (Ser726)\/STAT5B (Ser731) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-STAT5A (Ser726)\/STAT5B (Ser731) (80138-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-STAT5A (Ser726)\/STAT5B (Ser731) in WB, IF\/ICC, FC (Intra), ELISA applications with reactivity to human samples\n80138-2-RR targets Phospho-STAT5A (Ser726)\/STAT5B (Ser731) in WB, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: GM-CSF treated TF-1 cells\nPositive IF\/ICC detected in: GM-CSF treated TF-1 cells\nPositive FC (Intra) detected in: 100 nM Calyculin A (30 minutes) treated TF-1 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSTAT5 proteins consisting of STAT5A and STAT5B are the main targets of IL-3 signaling. Upon cytokine stimulation, JAK2 phosphorylates STAT5 and the phosphorylated STAT5 proteins dimerize and translocate into the nucleus, where, by binding DNA, they activate target genes including c-fos. In addition to this JAK-catalyzed tyrosine phosphorylation, STAT5 may undergo serine phosphorylation in the carboxy-terminal P(M)SP site in response to prolactin. (PMID: 15795318)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: signal transducer and activator of transcription 5A\nCalculated Molecular Weight: 794 aa, 92 kDa\nObserved Molecular Weight: 90-95 kDa\nGenBank Accession Number: BC027036\nGene Symbol: STAT5A\nGene ID (NCBI): 6776\nRRID: AB_3670468\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purfication\nUNIPROT ID: P42229\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888534966441,"sku":"80138-2-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-80138-2-rr","provider":"Iright","version":"1.0","type":"link"}