{"product_id":"proteintech-80144-2-rr","title":"Proteintech, 80144-2-RR, Phospho-SMAD9 (Ser465\/467) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-SMAD9 (Ser465\/467) (80144-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-SMAD9 (Ser465\/467) in WB, ELISA, ChIP-qPCR applications with reactivity to human samples\n80144-2-RR targets Phospho-SMAD9 (Ser465\/467) in WB, ELISA, ChIP-qPCR applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: BMP2 treated HeLa cells\nPositive ChIP-qPCR detected in: BMP2 (50 ng\/mL) for 1 h HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nCHIP-QPCR: CHIP-QPCR : 1:10-1:100\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMothers against decapentaplegic homolog 9 also known as SMAD9, SMAD8, and MADH6, belongs to the SMAD family of proteins, which belong to the TGFβ superfamily of modulators. Like many other TGFβ family members, SMAD9 is involved in cell signalling. When a bone morphogenetic protein binds to a receptor (BMP type 1 receptor kinase) it causes SMAD9 to interact with SMAD anchor for receptor activation (SARA).The binding of ligands causes the phosphorylation of the SMAD9 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD9 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase. There are two isoforms of the protein. The antibody recognizes both SMAD1 and SMAD5 protein phosphorylated at Ser463\/465.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: SMAD family member 9\nCalculated Molecular Weight: 430 aa, 49 kDa\nObserved Molecular Weight: 55 kDa\nGenBank Accession Number: BC011559\nGene Symbol: SMAD9\nGene ID (NCBI): 4093\nRRID: AB_3670469\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purfication\nUNIPROT ID: O15198\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888656437417,"sku":"80144-2-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-80144-2-rr","provider":"Iright","version":"1.0","type":"link"}