{"product_id":"proteintech-80281-2-rr","title":"Proteintech, 80281-2-RR, Phospho-ACC1 (Ser79) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-ACC1 (Ser79) (80281-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-ACC1 (Ser79) in WB, IF\/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples\n80281-2-RR targets Phospho-ACC1 (Ser79) in WB, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: λ phosphatase treated NIH\/3T3 cells, NIH\/3T3 cells\nPositive IF\/ICC detected in: Calyculin A treated HeLa cells\nPositive FC (Intra) detected in: Calyculin A treated HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:250-1:1000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nACC1 represents a key enzyme, as it is highly regulated by phosphorylation and allosteric regulation, providing a rapid adaptation to new micro-environmental conditions. AMPK phosphorylates acetyl CoA carboxylase (ACC), a rate-controlling step in the conversion of acetyl-CoA to malonyl CoA. This phosphorylation inhibits the activity of ACC, which results in decreased malonyl CoA levels. Additionally, two isoforms of ACC encoded by two different genes in mammalian cells have been described, ACC1 and ACC2. ACC1 is highly enriched in lipogenic tissues (liver and adipose), while ACC2 is mainly expressed in oxidative tissues (heart, skeletal muscle and liver). (PMID: 29056512, PMID: 16054041, PMID: 30816537)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: acetyl-Coenzyme A carboxylase alpha\nCalculated Molecular Weight: 2383 aa, 275 kDa\nObserved Molecular Weight: 250-270 kDa\nGenBank Accession Number: BC137287\nGene Symbol: Acetyl-CoA Carboxylase 1\nGene ID (NCBI): 31\nRRID: AB_3670473\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purfication\nUNIPROT ID: Q13085\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888868413609,"sku":"80281-2-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-80281-2-rr","provider":"Iright","version":"1.0","type":"link"}