{"product_id":"proteintech-80816-1-rr","title":"Proteintech, 80816-1-RR, AKT1-Specific Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe AKT1-Specific (80816-1-RR) by Proteintech is a Recombinant antibody targeting AKT1-Specific in WB, IHC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples\n80816-1-RR targets AKT1-Specific in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, MCF-7 cells,  HeLa cells,  A549 cells,  Jurkat cells,  K-562 cells,  NIH\/3T3 cells,  RAW 264.7 cells,  HSC-T6 cells,  PC-12 cells\nPositive IP detected in: HEK-293 cells\nPositive IHC detected in: human ovary tumor tissue, mouse small intestine tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive FC (Intra) detected in: Jurkat cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine\/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. 80816-1-RR recognizes AKT1 specifically.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag0213 Product name: Recombinant human AKT protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-224 aa of BC000479 Sequence: MSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDVDQREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQTVADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDRLC Predict reactive species\nFull Name: v-akt murine thymoma viral oncogene homolog 1\nCalculated Molecular Weight: 56 kDa\nObserved Molecular Weight: 56-62 kDa\nGenBank Accession Number: BC000479\nGene Symbol: AKT1\nGene ID (NCBI): 207\nRRID: AB_2935546\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P31749\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888342225065,"sku":"80816-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-80816-1-rr","provider":"Iright","version":"1.0","type":"link"}