{"product_id":"proteintech-81196-1-rr","title":"Proteintech, 81196-1-RR, ACSL4\/FACL4 Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe ACSL4\/FACL4 (81196-1-RR) by Proteintech is a Recombinant antibody targeting ACSL4\/FACL4 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples\n81196-1-RR targets ACSL4\/FACL4 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HepG2 cells,  L02 cells,  U-87 MG cells,  HT-29 cells,  NIH\/3T3 cells,  HSC-T6 cells\nPositive IP detected in: HeLa cells\nPositive IHC detected in: human liver cancer tissue, human stomach cancer tissue,  rat brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nAcyl-CoA synthetase long-chain family member 4 (Acsl4), an important enzyme involved in lipid metabolism, participates in ferroptosis by converting free AA into arachidonoyl-CoA to generate lipid hydroperoxides. Recent studies revealed that ACSL4 is involved in biological responses including inflammation, steroidogenesis, cell death, female fertility, and cancer. Acsl4 has two isoforms: 79 kDa and 75 kDa. ACSL4 also can be detected in 70 kDa(PMID:35326233).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag18085 Product name: Recombinant human ACSL4 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 143-236 aa of BC034959 Sequence: LGKEAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAINKAEYPEGFEIHSMQSVEELGSNPENLGIPPSRPTPSDMAIVMY Predict reactive species\nFull Name: acyl-CoA synthetase long-chain family member 4\nCalculated Molecular Weight: 711 aa, 79 kDa\nObserved Molecular Weight: 70 kDa, 75 kDa\nGenBank Accession Number: BC034959\nGene Symbol: ACSL4\nGene ID (NCBI): 2182\nRRID: AB_2923707\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: O60488\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888342585513,"sku":"81196-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-81196-1-rr","provider":"Iright","version":"1.0","type":"link"}