{"product_id":"proteintech-82371-5-rr","title":"Proteintech, 82371-5-RR, Phospho-GSK3A (Tyr279)\/GSK3B (Tyr216) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-GSK3A (Tyr279)\/GSK3B (Tyr216) (82371-5-RR) by Proteintech is a Recombinant antibody targeting Phospho-GSK3A (Tyr279)\/GSK3B (Tyr216) in WB, FC (Intra), ELISA applications with reactivity to human, mouse samples\n82371-5-RR targets Phospho-GSK3A (Tyr279)\/GSK3B (Tyr216) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, NIH\/3T3 cells\nPositive FC (Intra) detected in: λ phosphatase treated HEK-293 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nGlycogen synthase kinase 3 (GSK3) proteins are serine\/threonine kinases that were originally identified as key regulatory enzymes in glucose metabolism. There are two isoforms, GSK3 alpha and GSK3 beta, that are encoded by separate genes and that are 85% homologous to each other overall with 95% homology in the kinase domains. GSK3 beta is activated by phosphorylation of the tyrosine 216 residue (Tyr216) located in the kinase domain and inactivated by phosphorylation of the amino-terminal serine 9 residue (Ser9). GSK3 phosphorylation inhibits its catalytic activity towards its substrates, allowing cytosolic stabilization and nuclear translocation of the transcriptional targets, β-catenin and Nrf2. (PMID: 31354196 , PMID: 25750704,PMID: 24722055)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: glycogen synthase kinase 3 beta\nCalculated Molecular Weight: 433 aa, 48 kDa\nObserved Molecular Weight: 48-50 kDa\nGenBank Accession Number: BC000251\nGene Symbol: GSK3B\nGene ID (NCBI): 2932\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P49841\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888801566889,"sku":"82371-5-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-82371-5-rr","provider":"Iright","version":"1.0","type":"link"}