{"product_id":"proteintech-82508-1-rr","title":"Proteintech, 82508-1-RR, FH Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe FH (82508-1-RR) by Proteintech is a Recombinant antibody targeting FH in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples\n82508-1-RR targets FH in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HepG2 cells, HEK-293 cells,  A431 cells,  L02 cells,  mouse liver tissue,  rat liver tissue\nPositive IP detected in: mouse liver tissue\nPositive IHC detected in: human stomach tissue, human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:1000-1:4000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nFH(fumarate hydratase) belongs to the class-II fumarase\/aspartase family. It is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. FH also acts as a tumor suppressor function of FH in sporadic kidney cancer(PMID:21695080). Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag1934 Product name: Recombinant human FH protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 204-510 aa of BC003108 Sequence: HEVLLPGLQKLHDALDAKSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAMTRIKAAMPRIYELAAGGTAVGTGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGPK Predict reactive species\nFull Name: fumarate hydratase\nCalculated Molecular Weight: 510 aa, 55 kDa\nObserved Molecular Weight: 45 kDa\nGenBank Accession Number: BC003108\nGene Symbol: FH\nGene ID (NCBI): 2271\nRRID: AB_3086485\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P07954\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888355266729,"sku":"82508-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-82508-1-rr","provider":"Iright","version":"1.0","type":"link"}