{"product_id":"proteintech-82674-10-rr","title":"Proteintech, 82674-10-RR, Phospho-STAT1 (Tyr701) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-STAT1 (Tyr701) (82674-10-RR) by Proteintech is a Recombinant antibody targeting Phospho-STAT1 (Tyr701) in WB, FC (Intra), ELISA, ChIP-qPCR applications with reactivity to human samples\n82674-10-RR targets Phospho-STAT1 (Tyr701) in WB, FC (Intra), ELISA, ChIP-qPCR applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: IFN gamma treated THP-1 cells\nPositive FC (Intra) detected in: IFN gamma treated THP-1 cells\nPositive ChIP-qPCR detected in: IFN-γ (50 ng\/ml, 30min) HT-1080 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension\nCHIP-QPCR: CHIP-QPCR : 1:10-1:100\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSTAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. STAT family members are phosphorylated by the receptor associated kinases, and then form homo-or heterodimers that translocate to the cell nucleus where they act as transcription activators. The phosphorylation of STAT1 at Tyr701 promotes the dimerization of STAT1 and its translocation into the nucleus where it binds the GAS promoter to allow the transcription of inflammatory response genes(PMID: 31408005). The transcript of STAT1 undergoes alternative splicing, resulting in two isoforms: STAT1α (91 kDa) and STAT1β (84 kDa)(PMID: 20159032).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: signal transducer and activator of transcription 1, 91kDa\nCalculated Molecular Weight: 83 kDa\nObserved Molecular Weight: 84-91 kDa\nGenBank Accession Number: BC002704\nGene Symbol: STAT1\nGene ID (NCBI): 6772\nRRID: AB_3085362\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P42224\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888656634025,"sku":"82674-10-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-82674-10-rr","provider":"Iright","version":"1.0","type":"link"}