{"product_id":"proteintech-82738-4-rr","title":"Proteintech, 82738-4-RR, TOP2A Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe TOP2A (82738-4-RR) by Proteintech is a Recombinant antibody targeting TOP2A in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human samples\n82738-4-RR targets TOP2A in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: K-562 cells, HepG2 cells,  HeLa cells,  HEK-293 cells,  A431 cells,  Jurkat cells\nPositive IP detected in: HeLa cells\nPositive IHC detected in: human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: A431 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:2000-1:8000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:250-1:1000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nTOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL\/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a\/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag19551 Product name: Recombinant human TOP2A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1187-1531 aa of BC140791 Sequence: QDEQVGLPGKGGKAKGKKTQMAEVLPSPRGQRVIPRITIEMKAEAEKKNKKKIKNENTEGSPQEDGVELEGLKQRLEKKQKREPGTKTKKQTTLAFKPIKKGKKRNPWSDSESDRSSDESNFDVPPRETEPRRAATKTKFTMDLDSDEDFSDFDEKTDDEDFVPSDASPPKTKTSPKLSNKELKPQKSVVSDLEADDVKGSVPLSSSPPATHFPDETEITNPVPKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDDSDSNFEKIVSKAVTSKKSKGESDDFHMDFDSAVAPRAKSVRAKKPIKYLEESDEDDLF Predict reactive species\nFull Name: topoisomerase (DNA) II alpha 170kDa\nCalculated Molecular Weight: 1531 aa, 174 kDa\nObserved Molecular Weight: ~170 kDa\nGenBank Accession Number: BC140791\nGene Symbol: TOP2A\nGene ID (NCBI): 7153\nRRID: AB_3086521\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P11388\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888361984169,"sku":"82738-4-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-82738-4-rr","provider":"Iright","version":"1.0","type":"link"}