{"product_id":"proteintech-82762-11-rr","title":"Proteintech, 82762-11-RR, Phospho-HER2\/ErbB2 (Tyr877) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-HER2\/ErbB2 (Tyr877) (82762-11-RR) by Proteintech is a Recombinant antibody targeting Phospho-HER2\/ErbB2 (Tyr877) in WB, IF\/ICC, FC (Intra), ELISA applications with reactivity to human samples\n82762-11-RR targets Phospho-HER2\/ErbB2 (Tyr877) in WB, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: EGF treated A431 cells\nPositive IF\/ICC detected in: EGF treated A431 cells\nPositive FC (Intra) detected in: EGF treated A431 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:500-1:2000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nHER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein that is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. Phospho-HER2\/ErbB2 (Tyr877) refers to the phosphorylated form of the human epidermal growth-factor receptor 2 (HER2, ErbB2) at tyrosine 877 within its kinase domain. This phosphorylation event is catalyzed in trans by dimerization-activated HER2 itself or by Src-family kinases (SFKs) upon receptor clustering, and serves as a critical activation switch for downstream signaling cascades. ( PMID: 34107301)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro\/glioblastoma derived oncogene homolog (avian)\nCalculated Molecular Weight: 1255 aa, 138 kDa\nObserved Molecular Weight: 185 kDa\nGenBank Accession Number: BC156755\nGene Symbol: HER2\/ErbB2\nGene ID (NCBI): 2064\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P04626\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888801796265,"sku":"82762-11-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-82762-11-rr","provider":"Iright","version":"1.0","type":"link"}