{"product_id":"proteintech-82825-2-rr","title":"Proteintech, 82825-2-RR, Mono-Methyl-Histone H3 (Lys36) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Mono-Methyl-Histone H3 (Lys36) (82825-2-RR) by Proteintech is a Recombinant antibody targeting Mono-Methyl-Histone H3 (Lys36) in WB, IF\/ICC, FC (Intra), Dot Blot, ELISA, ChIP-qPCR applications with reactivity to human, mouse, rat samples\n82825-2-RR targets Mono-Methyl-Histone H3 (Lys36) in WB, IF\/ICC, FC (Intra), Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293T cells, HeLa cells,  THP-1 cells,  K-562 cells,  mouse liver tissue,  NIH\/3T3 cells,  HSC-T6 cells\nPositive IF\/ICC detected in: HeLa cells\nPositive FC (Intra) detected in: HeLa cells\nPositive Dot Blot detected in: peptide\nPositive ChIP-qPCR detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\nDOT BLOT: DOT BLOT : 1:10-1:100\nCHIP-QPCR: CHIP-QPCR : 1:10-1:100\n\u003cb\u003eBackground Information\u003c\/b\u003e\nHistones, including H1\/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: histone cluster 1, H3a\nObserved Molecular Weight: 15 kDa\nGenBank Accession Number: BC066245\nGene Symbol: HIST1H3A\nGene ID (NCBI): 8350\nRRID: AB_3670565\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purfication\nUNIPROT ID: P68431\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888435417257,"sku":"82825-2-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-82825-2-rr","provider":"Iright","version":"1.0","type":"link"}