{"product_id":"proteintech-83212-4-rr","title":"Proteintech, 83212-4-RR, NCBP1\/CBP80 Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe NCBP1\/CBP80 (83212-4-RR) by Proteintech is a Recombinant antibody targeting NCBP1\/CBP80 in WB, IHC, IF\/ICC, FC (Intra), ELISA applications with reactivity to human, rat samples\n83212-4-RR targets NCBP1\/CBP80 in WB, IHC, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Jurkat cells, HEK-293 cells,  HeLa cells,  HepG2 cells,  A431 cells,  NCI-H1299 cells,  PC-12 cells\nPositive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\nPositive FC (Intra) detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:200-1:800\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nNuclear cap binding protein subunit 1, 80kDa (NCBP1, synonyms: NCBP, CBP80) is a subunit of  a cap-binding protein complex (CBC) that consists of a CBP20 subunit, which binds the cap, and a NCBP1 subunit, which ensures high-affinity cap binding. The CBC complex  may play a role in pre-mRNA recognition and splicing.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag0496 Product name: Recombinant human CBP80 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 46-350 aa of BC001450 Sequence: LESNLEGLAGVLEADLPNYKSKILRLLCTVARLLPEKLTIYTTLVGLLNARNYNFGGEFVEAMIRQLKESLKANNYNEAVYLVRFLSDLVNCHVIAAPSMVAMFENFVSVTQEEDVPQVRRDWYVYAFLSSLPWVGKELYEKKDAEMDRIFANTESYLKRRQKTHVPMLQVWTADKPHPQEEYLDCLWAQIQKLKKDRWQERHILRPYLAFDSILCEALQHNLPPFTPPPHTEDSVYPMPRVIFRMFDYTDDPEGPVMPGSHSVERFVIEENLHCIIKSHWKERKTCAAQLVSYPGKNKIPLNYH Predict reactive species\nFull Name: nuclear cap binding protein subunit 1, 80kDa\nCalculated Molecular Weight: 92 kDa\nObserved Molecular Weight: 80 kDa\nGenBank Accession Number: BC001450\nGene Symbol: NCBP1\nGene ID (NCBI): 4686\nRRID: AB_3670896\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q09161\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888436531369,"sku":"83212-4-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-83212-4-rr","provider":"Iright","version":"1.0","type":"link"}