{"product_id":"proteintech-83735-6-rr","title":"Proteintech, 83735-6-RR, FOXK2 Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe FOXK2 (83735-6-RR) by Proteintech is a Recombinant antibody targeting FOXK2 in WB, IHC, IF\/ICC, FC (Intra), ELISA applications with reactivity to human samples\n83735-6-RR targets FOXK2 in WB, IHC, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293T cells, HAP1 cells,  HeLa cells,  MCF-7 cells,  T-47D cells. MDA-MB-231 cells,  U2OS cells\nPositive IHC detected in: human placenta tissue, human colon tissue,  human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: A431 cells\nPositive FC (Intra) detected in: HEK-293T cells, SH-SY5Y cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:250-1:1000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nFOXK2 (forkhead box K2), also known as ILF and ILF1. FOXK2 is localized at nucleus and cytoplasm. It is also expected to be expressed in testis, brain and lymphoid and non-lymphoid cells (PMID: 1339390). The protein encoded by this gene contains a fork head DNA binding domain. This protein can bind to the purine-rich motifs of the HIV long terminal repeat (LTR), and to the similar purine-rich motif in the interleukin 2 (IL2) promoter. It may be involved in the regulation of viral and cellular promoter elements. And its molecular weight is 69 kDa. Anett Marais demonstrate that FOXK2 is phosphorylated in a cell cycle-dependent manner. This phosphorylation peaks during M phase and is mediated by CDK·cyclin complexes. FOXK2 exhibits a periodic rise in its phosphorylation levels during the cell cycle, with hyperphosphorylation occurring in mitotic cells (PMID: 20810654).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag33625 Product name: Recombinant human FOXK2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 430-660 aa of NM_004514.3 Sequence: LSSQPVLITVQRQLPQAIKPVTYTVATPVTTSTSQPPVVQTVHVVHQIPAVSVTSVAGLAPANTYTVSGQAVVTPAAVLAPPKAEAQENGDHREVKVKVEPIPAIGHATLGTASRIIQTAQTTPVQTVTIVQQAPLGQHQLPIKTVTQNGTHVASVPTAVHGQVNNAAASPLHMLATHASASASLPTKRHNGDQPEQPELKRIKTEDGEGIVIALSVDTPPAAVREKGVQN* Predict reactive species\nFull Name: forkhead box K2\nCalculated Molecular Weight: 69 kDa\nObserved Molecular Weight: 69-79 kDa\nGenBank Accession Number: NM_004514.3\nGene Symbol: FOXK2\nGene ID (NCBI): 3607\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purfication\nUNIPROT ID: Q01167\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888753529001,"sku":"83735-6-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-83735-6-rr","provider":"Iright","version":"1.0","type":"link"}