{"product_id":"proteintech-83858-3-rr","title":"Proteintech, 83858-3-RR, ASC\/TMS1 Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe ASC\/TMS1 (83858-3-RR) by Proteintech is a Recombinant antibody targeting ASC\/TMS1 in WB, IHC, ELISA applications with reactivity to mouse samples\n83858-3-RR targets ASC\/TMS1 in WB, IHC, ELISA applications and shows reactivity with mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: J774A.1 cells, mouse lung tissue,  mouse thymus tissue,  mouse spleen tissue\nPositive IHC detected in: mouse colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunohistochemistry (IHC): IHC : 1:250-1:1000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPYCARD (PYD and CARD domain containing), also known as ASC and CARD5. The calculated molecular weight of PYCARD is 21 kDa. It is located in cytosol, extracellular region and nucleus. PYCARD is also expressed in several structures, including alimentary system; brain; heart; hemolymphoid system gland; and urinary system. The gene enables identical protein binding activity and protein dimerization activity. Involved in several processes, including activation of cysteine-type endopeptidase activity; positive regulation of cytokine production; and regulation of defense response. It acts upstream of or within several processes, including defense response to Gram-positive bacterium; positive regulation of macromolecule metabolic process; and regulation of autophagy. It is also a part of inflammasome complex. Finally, it orthologous to human PYCARD (PYD and CARD domain containing).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: mouse\nCited Reactivity: mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag32519 Product name: Recombinant mouse PYCARD protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-193 aa of NM_023258 Sequence: MGRARDAILDALENLSGDELKKFKMKLLTVQLREGYGRIPRGALLQMDAIDLTDKLVSYYLESYGLELTMTVLRDMGLQELAEQLQTTKEESGAVAAAASVPAQSTARTGHFVDQHRQALIARVTEVDGVLDALHGSVLTEGQYQAVRAETTSQDKMRKLFSFVPSWNLTCKDSLLQALKEIHPYLVMDLEQS Predict reactive species\nFull Name: PYD and CARD domain containing\nCalculated Molecular Weight: 21KD\nObserved Molecular Weight: 21-25 kDa\nGenBank Accession Number: NM_023258\nGene Symbol: Pycard\nGene ID (NCBI): 66824\nRRID: AB_3671442\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q9EPB4\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888530313385,"sku":"83858-3-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-83858-3-rr","provider":"Iright","version":"1.0","type":"link"}