{"product_id":"proteintech-84076-3-rr","title":"Proteintech, 84076-3-RR, ISLR2 Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe ISLR2 (84076-3-RR) by Proteintech is a Recombinant antibody targeting ISLR2 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n84076-3-RR targets ISLR2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse brain tissue, rat brain tissue\nPositive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nISLR2 (immunoglobulin superfamily containing leucine rich repeat 2), also known as LINX. It is expected to be located in cell membrane and the protein is mainly expressed in brain and testis. While the first knockout mouse had impaired development of the internal capsule and no hydrocephalus, a much more recent independent knockout mouse was shown to additionally have severe hydrocephalus. Although the mechanism remains unclear, it may be related to the role of ISLR2 in reorganizing the cytoskeleton of developing neurons (PMID: 30483960). The calculated molecular weight of ISLR2 is 79 kDa, this target has phosphorylation modification and glycosylation modification. And the 130 kDa band is glycosylated (PMID: 29739947).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag20505 Product name: Recombinant human ISLR2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 396-745 aa of BC152429 Sequence: ERKSTAKGRGNSVLPSKPEGKIKGQGLAKVSILGETETEPEEDTSEGEEAEDQILADPAEEQRCGNGDPSRYVSNHAFNQSAELKPHVFELGVIALDVAEREARVQLTPLAARWGPGPGGAGGAPRPGRRPLRLLYLCPAGGGAAVQWSRVEEGVNAYWFRGLRPGTNYSVCLALAGEACHVQVVFSTKKELPSLLVIVAVSVFLLVLATVPLLGAACCHLLAKHPGKPYRLILRPQAPDPMEKRIAADFDPRASYLESEKSYPAGGEAGGEEPEDVQGEGLDEDAEQGDPSGDLQREESLAACSLVESQSKANQEEFEAGSEYSDRLPLGAEAVNIAQEINGNYRQTAG Predict reactive species\nFull Name: immunoglobulin superfamily containing leucine-rich repeat 2\nCalculated Molecular Weight: 745 aa, 79 kDa\nObserved Molecular Weight: 79 kDa, 130 kDa\nGenBank Accession Number: BC152429\nGene Symbol: ISLR2\nGene ID (NCBI): 57611\nRRID: AB_3671645\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purfication\nUNIPROT ID: Q6UXK2\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888645558441,"sku":"84076-3-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-84076-3-rr","provider":"Iright","version":"1.0","type":"link"}