{"product_id":"proteintech-84192-4-rr","title":"Proteintech, 84192-4-RR, Ki-67 Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Ki-67 (84192-4-RR) by Proteintech is a Recombinant antibody targeting Ki-67 in WB, IHC, IF\/ICC, IF-P, ELISA applications with reactivity to human samples\n84192-4-RR targets Ki-67 in WB, IHC, IF\/ICC, IF-P, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HepG2 cells,  HEK-293 cells,  MCF-7 cells,  Ramos cells\nPositive IHC detected in: human tonsillitis tissue, human skin cancer tissue,  Human Spleen tissue,  Human renal cancer,  human malignant melanoma tissue,  human prostate cancer tissue,  human Breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: human lung cancer tissue, human thyroid cancer tissue\nPositive IF\/ICC detected in: HeLa cells, MCF-7 cells,  U2OS cells,  HepG2 cells,  A431 cells,  A549 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:1000-1:4000\nImmunofluorescence (IF)-P: IF-P : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: antigen identified by monoclonal antibody Ki-67\nCalculated Molecular Weight: 359 kDa\nObserved Molecular Weight: 359 kDa\nGenBank Accession Number: NM_002417\nGene Symbol: KI67\nGene ID (NCBI): 4288\nRRID: AB_3671744\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purfication\nUNIPROT ID: P46013\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888339538089,"sku":"84192-4-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-84192-4-rr","provider":"Iright","version":"1.0","type":"link"}