{"product_id":"proteintech-85175-5-rr","title":"Proteintech, 85175-5-RR, Cleaved PARP1 (Asp214) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Cleaved PARP1 (Asp214) (85175-5-RR) by Proteintech is a Recombinant antibody targeting Cleaved PARP1 (Asp214) in WB, IF\/ICC, ELISA applications with reactivity to human samples\n85175-5-RR targets Cleaved PARP1 (Asp214) in WB, IF\/ICC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Staurosporine treated HeLa cells, Staurosporine treated Jurkat cells\nPositive IF\/ICC detected in: Staurosporine treated Jurkat cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:400-1:1600\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24 kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes, and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and give rise to fragments ranging from 42-89 kDa.This antibody only recognizes the cleaved form of PAPR1 but not full-length PARP1.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: poly (ADP-ribose) polymerase 1\nCalculated Molecular Weight: 1014 aa, 113 kDa\nObserved Molecular Weight: 89 kDa\nGenBank Accession Number: BC037545\nGene Symbol: PARP1\nGene ID (NCBI): 142\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P09874\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888606105769,"sku":"85175-5-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-85175-5-rr","provider":"Iright","version":"1.0","type":"link"}