{"product_id":"proteintech-86456-1-rr","title":"Proteintech, 86456-1-RR, Entactin Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Entactin (86456-1-RR) by Proteintech is a Recombinant antibody targeting Entactin in WB, IHC, IF-P, ELISA applications with reactivity to mouse, rat samples\n86456-1-RR targets Entactin in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse heart tissue, rat heart tissue,  mouse skeletal muscle tissue\nPositive IHC detected in: mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: mouse heart tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:2000-1:8000\nImmunofluorescence (IF)-P: IF-P : 1:250-1:1000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nNidogen 1 (Entactin), first identified from a basement membrane-secreting cell line and the murine EHS tumor, is together with perlecan, laminin, and collagen IV, a ubiquitous component of basement membranes (PMID: 19530222). Nidogen-1 is made by mesenchymal cells and is incorporated into the epithelial BM. It is a sulfated glycoprotein of 150 kDa with three globular domains, G1, G2 and G3, and contains binding sites for other ECM molecules (PMID: 10958695, 10671374).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Eg3030 Product name: Recombinant Mouse NID1 protein (rFc Tag) Source: mammalian cells -derived, V37 Tag: C-rFc Domain: 29-1245 aa of NM_010917.3 Sequence: LNRQELFPFGPGQGDLELEAGDDVVSPSLELIGELSFYDRTDITSVYVTTNGIIAMSEPPATEYHPGTFPPSFGSVAPFLADLDTTDGLGNVYYREDLSPFIIQMAAEYVQRGFPEVSFQPTSVVVVTWESVAPYGGPSSSPAEEGKRNTFQAVLASSNSSSYAIFLYPEDGLQFFTTFSKKDESQVPAVVGFSKGLVGFLWKSNGAYNIFANDRESIENLAKSSNAGHQGVWVFEIGSPATAKGVVSADVNLDLDDDGADYEDEDYDLVTSHLGLEDVATPSPSHSPRRGYPDPHNVPRILSPGYEATERPRGVPTERTRSFQLPAERFPQHHPQVIDVDEVEETGVVFSYNTGSQQTCANNRHQCSVHAECRDYATGFCCRCVANYTGNGRQCVAEGSPQRVNGKVKGRIFVGSSQVPVVFENTDLHSYVVMNHGRSYTAISTIPETVGYSLLPLAPIGGIIGWMFAVEQDGFKNGFSITGGEFTRQAEVTFLGHPGKLVLKQQFSGIDEHGHLTISTELEGRVPQIPYGASVHIEPYTELYHYSSSVITSSSTREYTVMEPDQDGAAPSHTHIYQWRQTITFQECAHDDARPALPSTQQLSVDSVFVLYNKEERILRYALSNSIGPVRDGSPDALQNPCYIGTHGCDSNAACRPGPGTQFTCECSIGFRGDGQTCYDIDECSEQPSRCGNHAVCNNLPGTFRCECVEGYHFSDRGTCVAAEDQRPINYCETGLHNCDIPQRAQCIYMGGSSYTCSCLPGFSGDGRACRDVDECQHSRCHPDAFCYNTPGSFTCQCKPGYQGDGFRCMPGEVSKTRCQLEREHILGAAGGADAQRPTLQGMFVPQCDEYGHYVPTQCHHSTGYCWCVDRDGRELEGSRTPPGMRPPCLSTVAPPIHQGPVVPTAVIPLPPGTHLLFAQTGKIERLPLERNTMKKTEAKAFLHIPAKVIIGLAFDCVDKVVYWTDISEPSIGRASLHGGEPTTIIRQDLGSPEGIALDHLGRTIFWTDSQLDRIEVAKMDGTQRRVLFDTGLVNPRGIVTDPVRGNLYWTDWNRDNPKIETSHMDGTNRRILAQDNLGLPNGLTFDAFSSQLCWVDAGTHRAECLNPAQPGRRKVLEGLQYPFAVTSYGKNLYYTDWKTNSVIAMDLAISKEMDTFHPHKQTRLYGITIALSQCPQGHNYCSVNNGGCTHLCLPTPGSRTCRCPDNTLGVDCIERK Predict reactive species\nFull Name: nidogen 1\nCalculated Molecular Weight: 137 kDa\nObserved Molecular Weight: 150 kDa\nGenBank Accession Number: NM_010917.3\nGene Symbol: Nid1\nGene ID (NCBI): 18073\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P10493\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888739995817,"sku":"86456-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-86456-1-rr","provider":"Iright","version":"1.0","type":"link"}