{"product_id":"proteintech-86644-1-rr","title":"Proteintech, 86644-1-RR, Alix Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Alix (86644-1-RR) by Proteintech is a Recombinant antibody targeting Alix in WB, IF\/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples\n86644-1-RR targets Alix in WB, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, NIH\/3T3 cells,  HSC-T6 cells,  HEK-293T cells,  PANC-1 cells,  Jurkat cells\nPositive IF\/ICC detected in: HeLa cells\nPositive FC (Intra) detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 is a 22-kDa protein containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium. The alix antibody recognizes an additional band of 75-80 kDa which has also been observed in cells and exosomes.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag3074 Product name: Recombinant human ALIX; AIP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 515-868 aa of BC020066 Sequence: SHRDTIVLLCKPEPELNAAIPSANPAKTMQGSEVVNVLKSLLSNLDEVKKEREGLENDLKSVNFDMTSKFLTALAQDGVINEEALSVTELDRVYGGLTTKVQESLKKQEGLLKNIQVSHQEFSKMKQSNNEANLREEVLKNLATAYDNFVELVANLKEGTKFYNELTEILVRFQNKCSDIVFARKTERDELLKDLQQSIAREPSAPSIPTPAYQSSPAGGHAPTPPTPAPRTMPPTKPQPPARPPPPVLPANRAPSATAPSPVGAGTAAPAPSQTPGSAPPPQAQGPPYPTYPGYPGYCQMPMPMGYNPYAYGQYNMPYPPVYHQSPGQAPYPGPQQPSYPFPQPPQQSYYPQQ Predict reactive species\nFull Name: programmed cell death 6 interacting protein\nCalculated Molecular Weight: 868 aa, 96 kDa\nObserved Molecular Weight: 100 kDa\nGenBank Accession Number: BC020066\nGene Symbol: Alix\nGene ID (NCBI): 10015\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q8WUM4\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888691663017,"sku":"86644-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-86644-1-rr","provider":"Iright","version":"1.0","type":"link"}